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Kaneka Corp reverse transcriptase core kit
Reverse Transcriptase Core Kit, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+core+kit/core+kit+reverse+transcriptase/bio_rxiv__64898__2026__04__02__713107-119-16-20
Average 86 stars, based on 1 article reviews
reverse transcriptase core kit - by Bioz Stars, 2026-09
86/100 stars

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Reverse Transcription:

Article Title: HuR-Driven Reversible Mitochondrial Shuttling Buffers Cytosolic miRNA Levels in Hepatic Cells.
Article Snippet: .. To quantify mRNA, we prepared cDNA from RNA samples using the Eurogentec Reverse Transcriptase Core Kit. .. Real-time PCR was conducted on the cDNA utilizing the Mesa Green Jo urn al Pr -pr oo f 31 qPCR Mastermix Plus for SYBR Assay-Low ROX (Eurogentec).

Article Title: HuR-driven reversible mitochondrial shuttling buffers cytosolic miRNA levels in hepatic cells
Article Snippet: .. To quantify mRNA, we prepared cDNA from RNA samples using the Eurogentec Reverse Transcriptase Core Kit. .. Real-time PCR was conducted on the cDNA utilizing the Mesa Green qPCR Mastermix Plus for SYBR Assay-Low ROX (Eurogentec).

Article Title: Change of guards: ELAVL proteins switch miRNA export responsibility to regulate differentiation of PC12 cells.
Article Snippet: RNA Isolation and qRT-PCR Total RNA was extracted with TRIzol or TRIzol LS (Invitrogen). mRNA and miRNA levels were quantified via two-step qRT-PCR. .. For mRNA, 200 ng of RNA was reverse-transcribed using the Eurogentec Reverse Transcriptase Core Kit and amplified with MESA GREEN qPCR Master Mix. .. For miRNA, 200 ng RNA was analysed with TaqMan miRNA-specific primers (Invitrogen), using equal volumes for FH-HuD-associated and exosomal miRNAs.

Article Title: From bats to humans: uncovering ISG15 as a new resistance factor in type 1 diabetes
Article Snippet: .. Poly(A) + RNA was isolated using the Dynabeads mRNA DIRECT Kit (Invitrogen) and reverse-transcribed using the Reverse Transcriptase Core Kit (Eurogentec), following the manufacturer’s protocol. hIsMTs were pooled (6 per well), washed twice in PBS and transferred to PCR-clean 2 mL Eppendorf tubes. .. QIAzol lysis buffer (Qiagen, 79306) was added and hIsMTs were vortexed and sonicated to aid thorough lysis.

Article Title: Iron deficiency induces maturation-dependent loss of pancreatic β-cells.
Article Snippet: For EndoC-βH1 and iPSC-β-cells, poly(A)+-RNA was isolated using the Dynabeads mRNA DIRECT Purification kit (Invitrogen, 61012). .. Reverse transcription was done using the Reverse Transcriptase Core Kit (Eurogentec, RT-RTCK-03). .. Real-time qRT-PCR amplification was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725274) on the CFX Connect instrument (Bio-Rad) and compared to a standard curve.

Article Title: Lactate treatment improves brain biochemistry and cognitive function in transgenic Alzheimer’s and wild-type mice
Article Snippet: RNA quality and concentration were assessed with a Nanodrop 2000c spectrophotometer (Thermo Scientific, USA), ensuring the samples were kept on ice during handling. .. RNA concentrations were normalised to 38.2 ng/μL, and cDNA was synthesised using the Reverse Transcriptase Core Kit (Eurogentec, RT-RTCK-05; Lot. ..

Article Title: Iron deficiency induces maturation-dependent loss of pancreatic β-cells
Article Snippet: For EndoC-βH1 and iPSC-β-cells, poly(A) + -RNA was isolated using the Dynabeads mRNA DIRECT Purification kit (Invitrogen, 61012). .. Reverse transcription was done using the Reverse Transcriptase Core Kit (Eurogentec, RT-RTCK-03). .. Real-time qRT-PCR amplification was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725274) on the CFX Connect instrument (Bio-Rad) and compared to a standard curve.

Article Title: Change of guards: ELAVL proteins switch miRNA export responsibility to regulate differentiation of PC12 cells
Article Snippet: Total RNA was extracted with TRIzol or TRIzol LS (Invitrogen). mRNA and miRNA levels were quantified via two-step qRT-PCR. .. For mRNA, 200 ng of RNA was reverse-transcribed using the Eurogentec Reverse Transcriptase Core Kit and amplified with MESA GREEN qPCR Master Mix. .. For miRNA, 200 ng RNA was analysed with TaqMan miRNA-specific primers (Invitrogen), using equal amount of FH-HuD-associated and exosomal miRNAs.

Amplification:

Article Title: Change of guards: ELAVL proteins switch miRNA export responsibility to regulate differentiation of PC12 cells.
Article Snippet: RNA Isolation and qRT-PCR Total RNA was extracted with TRIzol or TRIzol LS (Invitrogen). mRNA and miRNA levels were quantified via two-step qRT-PCR. .. For mRNA, 200 ng of RNA was reverse-transcribed using the Eurogentec Reverse Transcriptase Core Kit and amplified with MESA GREEN qPCR Master Mix. .. For miRNA, 200 ng RNA was analysed with TaqMan miRNA-specific primers (Invitrogen), using equal volumes for FH-HuD-associated and exosomal miRNAs.

Article Title: Change of guards: ELAVL proteins switch miRNA export responsibility to regulate differentiation of PC12 cells
Article Snippet: Total RNA was extracted with TRIzol or TRIzol LS (Invitrogen). mRNA and miRNA levels were quantified via two-step qRT-PCR. .. For mRNA, 200 ng of RNA was reverse-transcribed using the Eurogentec Reverse Transcriptase Core Kit and amplified with MESA GREEN qPCR Master Mix. .. For miRNA, 200 ng RNA was analysed with TaqMan miRNA-specific primers (Invitrogen), using equal amount of FH-HuD-associated and exosomal miRNAs.

Real-time Polymerase Chain Reaction:

Article Title: Change of guards: ELAVL proteins switch miRNA export responsibility to regulate differentiation of PC12 cells.
Article Snippet: RNA Isolation and qRT-PCR Total RNA was extracted with TRIzol or TRIzol LS (Invitrogen). mRNA and miRNA levels were quantified via two-step qRT-PCR. .. For mRNA, 200 ng of RNA was reverse-transcribed using the Eurogentec Reverse Transcriptase Core Kit and amplified with MESA GREEN qPCR Master Mix. .. For miRNA, 200 ng RNA was analysed with TaqMan miRNA-specific primers (Invitrogen), using equal volumes for FH-HuD-associated and exosomal miRNAs.

Article Title: Change of guards: ELAVL proteins switch miRNA export responsibility to regulate differentiation of PC12 cells
Article Snippet: Total RNA was extracted with TRIzol or TRIzol LS (Invitrogen). mRNA and miRNA levels were quantified via two-step qRT-PCR. .. For mRNA, 200 ng of RNA was reverse-transcribed using the Eurogentec Reverse Transcriptase Core Kit and amplified with MESA GREEN qPCR Master Mix. .. For miRNA, 200 ng RNA was analysed with TaqMan miRNA-specific primers (Invitrogen), using equal amount of FH-HuD-associated and exosomal miRNAs.

Isolation:

Article Title: From bats to humans: uncovering ISG15 as a new resistance factor in type 1 diabetes
Article Snippet: .. Poly(A) + RNA was isolated using the Dynabeads mRNA DIRECT Kit (Invitrogen) and reverse-transcribed using the Reverse Transcriptase Core Kit (Eurogentec), following the manufacturer’s protocol. hIsMTs were pooled (6 per well), washed twice in PBS and transferred to PCR-clean 2 mL Eppendorf tubes. .. QIAzol lysis buffer (Qiagen, 79306) was added and hIsMTs were vortexed and sonicated to aid thorough lysis.

Polymerase Chain Reaction:

Article Title: From bats to humans: uncovering ISG15 as a new resistance factor in type 1 diabetes
Article Snippet: .. Poly(A) + RNA was isolated using the Dynabeads mRNA DIRECT Kit (Invitrogen) and reverse-transcribed using the Reverse Transcriptase Core Kit (Eurogentec), following the manufacturer’s protocol. hIsMTs were pooled (6 per well), washed twice in PBS and transferred to PCR-clean 2 mL Eppendorf tubes. .. QIAzol lysis buffer (Qiagen, 79306) was added and hIsMTs were vortexed and sonicated to aid thorough lysis.



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Kaneka Corp reverse transcriptase core kit
Reverse Transcriptase Core Kit, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+core+kit/core+kit+reverse+transcriptase/bio_rxiv__64898__2026__04__02__713107-119-16-20
Average 86 stars, based on 1 article reviews
reverse transcriptase core kit - by Bioz Stars, 2026-09
86/100 stars
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